Serveur d'exploration Phytophthora

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Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags.

Identifieur interne : 002180 ( Main/Exploration ); précédent : 002179; suivant : 002181

Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags.

Auteurs : Franck Panabières [France] ; Joëlle Amselem ; Eric Galiana ; Jo-Yanne Le Berre

Source :

RBID : pubmed:15950158

Descripteurs français

English descriptors

Abstract

Phytophthora parasitica is a soilborne oomycete pathogen capable of infecting a wide range of plants, including many solanaceous plants. In a first step towards large-scale gene discovery, we generated expressed sequence tags (ESTs) from a cDNA library constructed using mycelium grown in synthetic medium. A total of 3568 ESTs were assembled into 2269 contiguous sequences. Functional categorization could be performed for 65.45% of ESTs. A significant portion of the transcripts encodes proteins of common metabolic pathways. The most prominent sequences correspond to members of the elicitin family, and enzymes involved in the lipid metabolism. A number of genes potentially involved in pathogenesis were also identified, which may constitute virulence determinants.

DOI: 10.1016/j.fgb.2005.03.002
PubMed: 15950158


Affiliations:


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Le document en format XML

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<title xml:lang="en">Gene identification in the oomycete pathogen Phytophthora parasitica during in vitro vegetative growth through expressed sequence tags.</title>
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<nlm:affiliation>INRA UMR 1064, Unité Interactions Plantes-Microorganismes et Santé Végétale, 400 route des Chappes, F-06930 Sophia-Antipolis cedex, France. panab@antibes.inra.fr</nlm:affiliation>
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<name sortKey="Galiana, Eric" sort="Galiana, Eric" uniqKey="Galiana E" first="Eric" last="Galiana">Eric Galiana</name>
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<name sortKey="Le Berre, Jo Yanne" sort="Le Berre, Jo Yanne" uniqKey="Le Berre J" first="Jo-Yanne" last="Le Berre">Jo-Yanne Le Berre</name>
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<term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Expressed Sequence Tags (metabolism)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Library (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mycelium (genetics)</term>
<term>Mycelium (growth & development)</term>
<term>Mycelium (pathogenicity)</term>
<term>Phylogeny (MeSH)</term>
<term>Phytophthora (genetics)</term>
<term>Phytophthora (growth & development)</term>
<term>Phytophthora (pathogenicity)</term>
<term>Proteome (MeSH)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Transcription, Genetic (MeSH)</term>
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<term>Alignement de séquences (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Banque de gènes (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Mycelium (croissance et développement)</term>
<term>Mycelium (génétique)</term>
<term>Mycelium (pathogénicité)</term>
<term>Phylogenèse (MeSH)</term>
<term>Phytophthora (croissance et développement)</term>
<term>Phytophthora (génétique)</term>
<term>Phytophthora (pathogénicité)</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Protéome (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Transcription génétique (MeSH)</term>
<term>Étiquettes de séquences exprimées (métabolisme)</term>
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<term>Fungal Proteins</term>
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<term>Mycelium</term>
<term>Phytophthora</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Mycelium</term>
<term>Phytophthora</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en">
<term>Mycelium</term>
<term>Phytophthora</term>
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<term>Mycelium</term>
<term>Phytophthora</term>
<term>Protéines fongiques</term>
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<term>Expressed Sequence Tags</term>
<term>Fungal Proteins</term>
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<term>Protéines fongiques</term>
<term>Étiquettes de séquences exprimées</term>
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<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en">
<term>Mycelium</term>
<term>Phytophthora</term>
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<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr">
<term>Mycelium</term>
<term>Phytophthora</term>
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<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Gene Library</term>
<term>Genes, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Proteome</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, DNA</term>
<term>Transcription, Genetic</term>
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<term>Alignement de séquences</term>
<term>Analyse de séquence d'ADN</term>
<term>Banque de gènes</term>
<term>Données de séquences moléculaires</term>
<term>Gènes fongiques</term>
<term>Phylogenèse</term>
<term>Protéome</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Transcription génétique</term>
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<div type="abstract" xml:lang="en">Phytophthora parasitica is a soilborne oomycete pathogen capable of infecting a wide range of plants, including many solanaceous plants. In a first step towards large-scale gene discovery, we generated expressed sequence tags (ESTs) from a cDNA library constructed using mycelium grown in synthetic medium. A total of 3568 ESTs were assembled into 2269 contiguous sequences. Functional categorization could be performed for 65.45% of ESTs. A significant portion of the transcripts encodes proteins of common metabolic pathways. The most prominent sequences correspond to members of the elicitin family, and enzymes involved in the lipid metabolism. A number of genes potentially involved in pathogenesis were also identified, which may constitute virulence determinants.</div>
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